Panangiparaan tan Pankaukolan na Chicken Adrenomedullin (ADM) Reagent

1. Serum: panag-coagulate ed temperatura na kuarto ed loob na 10-20 min, panag-centrifugate ed loob na 20 min diad kapeles ya 2000-3000 r.pm. Mantipon na maong na supernatant tan i-centrifuge lamet no walay ompatnag ya uran legan ya isimpen.

2. Plasma: Say EDTA o sodium citrate so nepeg ya pilien bilang anticoagulant unong ed saray pankaukolan na specimen. Kayari na panlaok ed loob na 10-20 minutos, i-centrifuge ed loob na 20 minutos diad kapeles ya 2000-3000 r.pm. Mantipon na maong na supernatant tan i-centrifuge lamet no walay uran legan ya isimpen.

3. Ihi: kolektaen panamegley na sterile tube, i-centrifuge ed loob na 20 minutos diad kapeles ya 2000-3000 r.pm. Mantipon na maong na supernatant tan i-centrifuge lamet no walay uran legan ya isimpen. Say thoracic tan abdominal fluid, cerebrospinal fluid reference implementation.

4. Cell culture supernatant: kolektaen panamegley na sterile tube no nanengneng iray exudatory components. I-centrifuge ed loob na 20 minutos diad kapeles ya 2000-3000 r.pm. Mantipon na maong na supernatant. Nen nadetektaan iray kabiangan ed saray selula, say suspension na selula et inlaok na PBS (pH 7.2-7.4), tan say konsentrasyon na selula et akasabi ed manga 1 milyon/mL. Diad paulit-ulit ya panagyelo tan panagtunaw, naderal iray selula tan nibulos iray kabiangan na selula. I-centrifuge ed loob na 20 minutos diad kapeles ya 2000-3000 r.pm. Mantipon na maong na supernatant. No walay uran legan ya manpreserba, i-centrifuge lamet.

5. Tissue specimen: Kayari na panputot ed specimen, timbangen. Mangiyan na pigaran PBS, PH7.4. Maples ya cryopreservation ed likidon nitroheno parad pangusar ed saginonor. Say specimen et mansiansia ed temperaturan 2-8℃kayari na pantunaw. Walay pigaran PBS (PH7.4) ya inyarum, tan say specimen et sigpot ya in-homogenize panamegley na lima odino panamegley na homogenizer. I-centrifuge ed loob na 20 minutos diad kapeles ya 2000-3000 r.pm. Mantipon na maong na supernatant. Inapag so sakey parad panagsubok tan i-freeze so arum parad reserba.

6. Saray specimen et nepeg ya alaen ed sankasakbayan a panaon kayari na panagkolekta. Say panag-extract et nepeg ya gawaen unong ed literatura, tan saray eksperimento et nepeg ya gawaen ed sankasakbayan a panaon kayari na extraction. No ag nagawaan a tampol so test, nayarin isimpen so specimen ed -20 degree, balet nepeg ya paliisan so paulit-ulit ya panagyelo tan panagtunaw.

7. Ag nanengneng so sample ya walay NaN3 to, lapud say NaN3 et mangaamper ed aktibidad na HRP.