Porcine Circovirus Type-II Antibody ELISA Detection Kit
Model No:VD510E
Abbr.:PCV-II Ab Elisa
Specimen: serum
Deskripsyon
Espesipikasyon
PCV-II Ab Elisa
Gagalaen ya Usaren:
Say Porcine Circovirus Type-II Antibody ELISA Detection Kit et parad pakaamta na espisipikon PCV antibody ed serum na baboy diad vitro. Say test et uusaren pian nanengneng no siopa ray abakunaan manlapud panagbakuna na porcine circovirus type-II ya bakuna na baboy.
Specimen:serum
Saray Materyales ya Inter:
Saray Pamaakaran ed Pansubok tan Interpretasyon
STEP 1 Pamaakaran na Pansubok
1.1 Panagparaan na Solusyon na Pan-uras
Mankikimey ya konsentrasyon na likido ed panag-uras: Antis ya usaren, say solusyon ed panag-uras et nepeg ya ipawil ed temperatura na kuarto (20-25℃) tan yugyogen ya parepareho pian magmaliw ya parepareho. Insan i-dilute na 20 beses so concentrated wash solution ed puron danum (alimbawa, 5ml ya concentrated washing solution so iyarum ed 95ml ya purified ya danum), ilaok ya maong, tan say diluted washing liquid et nayarin isimpen ed 2-8℃ed loob na 7 agew.
1.2 Panagparaan na Sampol
Inala so interon dala na ayep, in-centrifuge ed 4000rpm ed loob na 10 minutos, tan inala so supernatant. Say serum et nayarin na-coagulate met ya natural ya anggapoy hemolysis.
Lasawen so serum sample na 1:25 (idagdag so 5ul serum sample ed 120ul sample diluent) tan ilaok ya maong.
Tandaan: Saray negatibo tan positibon kontrol et ag kaukolan ya i-dilute.
1.3 Panagparaan na enzyme conjugate
Lasawen so enzyme conjugate na 1:25 (idagdag so 5ul ya enzyme conjugate ed 120ul ya enzyme diluent) tan ilaok ya maong.
1.4 Pamaakaran na Pansubok
1Alaen so enzyme label ya Microplate (unong ed bilang na sample, nayarin i-disassemble tan usaren na pigaran danay), ikarga so 100ul na diluted serum ed enzyme label ya Microplate, mangiyan na duaran well kada sakey parad negatibo tan positibo iran sample, tan idagdag so 100ul kada sakey parad negatibo tan positibon kontrol ed reaction well. Maalwar ya yugyogen so sample ed bobon, ya usaren so sealing plate film pian sakbongan so plato, tan i-incubate ed 37℃ed loob na 30 minutos.
Lukasan so sealing plate film, yugyogen so solusyon ed plate well, idagdag so 300μl na working concentration washing liquid ed kada well, insan ibantak so likido ed well. Uliten iray kundang ed tagey na mamin-5, insan diad kaunoran et pamaraan ed malinis ya absorbent paper.
3Ilaktip so 100μl na Enzyme Conjugate ed kada bobon, takloban so plato, tan i-incubate ed 37℃ed loob na 45 minutos.
4Luksan so sealing plate film, yugyogen so solusyon ed plate well, idagdag so 300μl na working concentration washing liquid ed kada well, insan ibantak so likido ed well. Uliten iray kundang ed tagey na mamin-5, insan diad kaunoran et pamaraan ed malinis ya absorbent paper.
⑤Ilaktip so 100μl ya substrate solution ed kada bobon, takloban so plato tan i-incubate ed loob na 10 minutos ed 37℃ya arawi ed liwawa.
6Idagdag so 50μl ya stop solution ed kada bobon, tan amtaen so resulta ed loob na 5 minuto.
STEP 2 Resulta Interpretation
Say OD450 ya balor na kada bobon et nabasa ed 96-well microplate reader.
2.1 Panangipatnag
Say average ya OD450nm ya balor na negatibon kontrol et<0.1 and the average OD450nm value of positive control /the average OD450nm value of negative control was ≥5, the experimental results were valid. Otherwise, the test should be repeated.
2.2 Paraan na Pankuenta
Parad kada sample, kalkulaen so S/P unong ed onggendan ya usaren so inkorehi ya sample tan control values:
S / P=(ODPANGALIGAN-ODNC) / (ODPC-ODNC)
2.3 Pangipaliwawa na Resulta
No S/P<0.15, it is judged as negative.
No say S/P et mas baleg odino mipara ed 0.25, ipasen ya positibo.
No 0.15 Mas abeba o mipara ed S/P<0.25, it is judged as suspicious, and suggest to repeat sampling and test one week later.
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Akaunan: Wala ray Impormasyon .
